江苏大学学报（医学版）2012，Vol.22Issue(2)：97-100,4.
转染CTGF基因对乳腺癌细胞MMP-2及TIMP-2表达的影响
Effect of gene CTGF transfection on the expression of MMP-2 and TIMP-2 in human breast cancer cells
摘要
Abstract
Objective: To investigate the effect of connective tissue growth factor ( CTGF) on the expression of matrix metalloproteinase -2 ( MMP-2 ) and tissue inhibitor of metalloproteinase -2 ( TIMP-2 ) in human breast cancer line MCF-7. This study was designed to explore the underlying mechanism for the role of CT -GF in the development of breast cancer. Methods; Lipofectin method was used to transfect CTGF vectors into MCF-7; immunofluorescence , RT-PCR and Western blot analysis were used to detect their transfection efficiency. The expressions of MMP-2 and TIMP-2 were determined by Western blot analysis. Results; Immunofluorescence , RT-PCR and Western blot analysis showed a successful transfection of CTGF vectors into MCF-7. MCF-7 transfected with CTGF gene showed an increased expression of MMP-2 protein significantly, compared with control groups. On the other hand, MCF-7 transfected with CTGF gene showed a decreased expression of TIMP-2 protein significantly, compared with control groups. Conclusion; It is possible that CTGF could promote the development of breast cancer by effectively enhancing the expressions of MMP -2 and TIMP-2 in MCF-7 cells.关键词
结缔组织生长因子/基质金属蛋白酶-2/基质金属蛋白酶组织抑制因子-2Key words
connective tissue growth factor/matrix metalloproteinase-2/tissue inhibitor of metalloproteinase-2分类
医药卫生引用本文复制引用
许斌,陈寅,戴桂红,蒋小芹,王薇,赵国军,于鸿..转染CTGF基因对乳腺癌细胞MMP-2及TIMP-2表达的影响[J].江苏大学学报（医学版）,2012,22(2):97-100,4.基金项目
南通大学自然科学基金资助项目(10Z088) （10Z088）
泰州市"311工程"人才基金项目(201036) （201036）
