江苏大学学报（医学版）2012，Vol.22Issue(2)：110-113,119,5.
外源性一氧化碳释放分子对脓毒症小鼠神经系统抗炎的调节作用
Regulation of carbon monoxide-releasing molecule-2 on inflammatory responses in nervous system of septic mice
摘要
Abstract
Objective; To investigate the regulation effects of exogenous carbon monoxide- releasing mol-ecule-2 ( CORM-2) on the nervous system in septic mice. Methods; To make standard cecal ligation and puncture (CLP) models, 48 C57BL/6 mice were randomly divided into 4 groups; sham group, CLP group, CORM-2 intervention group and Carbachol group. The animals were sacrificed at 24 hours after experimental manipulation. Serum and brain tissue samples were collected ,the expression of TNF-α, IL-6, neural expression of anti inflammatory neurotransmitter acetylcholine ( Ach) , and the acetylcholinesterase ( AChE) activity of brain tissues. To measure the wet-to-dry weight ratio of brain tissues and the pathology changes of brain tissues were also observed by HE dyeing. Results; Compared with the CLP mice , tissues injuries, pro-inflammatory cytokines ( TNF-α and IL-6 ) expressions were markedly down regulated in CORM -2 and Carbachol intervention groups ; while the concentration of neurotransmitter Ach were increased , and the activity of AChE were inhibited in CORM-2 and Carbachol intervention mice. Conclusion; Taken together, the data of this study showed that CORM -2 intervention markedly inhibited inflammatory responses in the nervous system, indicating that the CORM-2 may play an important roles in the treatment of sepsis , and will be useful for the clinical treatment of critically ill patients in the future .关键词
脓毒症/一氧化碳/乙酰胆碱/胆碱能抗炎通路Key words
sepsis/carbon monoxide/acetylcholine/cholinergic antiinflammatory pathway分类
医药卫生引用本文复制引用
仇雪枫,孙炳伟,王敏,孙艳,杨国涛,王波..外源性一氧化碳释放分子对脓毒症小鼠神经系统抗炎的调节作用[J].江苏大学学报（医学版）,2012,22(2):110-113,119,5.基金项目
国家自然科学基金资助项目(30772256,81071546) （30772256,81071546）
江苏省自然科学基金资助项目(BK2008237) （BK2008237）
