江苏大学学报（医学版）2012，Vol.22Issue(2)：137-140,150,5.
吗啡对乳腺癌MCF-7细胞生存素及livin表达的影响
Effect of morphine on the expression of survivin and livin in human breast cancer cells
摘要
Abstract
Objective; To investigate the effect of morphine on proliferation and apoptosis in human breast cancer cell line MCF-7, with a focus on the expression of survivin and livin . MCF-7 cells were seeded in 6-well plates(1 ×103 /ml) and divided into 3 groups; group I normal control(group C) , group Ⅱ was exposed to 0.1 μmol/L morphine ( group Ml ) , group Ⅲ was exposed to 1. 0 μmol/L morphine ( group M2). The expression of survivin and livin in different group of cells was detected by RT -PCR and Western blot at 24 hours of incubation with morphine. The apoptosis of cells was determined by flow cytometry at 24 hours of incubation with morphine. The proliferation of cells was determined by Thiazolyl blue tetrazolium bromide (MTT) method at 24,48, and 72 hours of incubation with morphine respectively. Results; Compared with group C, the expression of survivin and livin , and the proliferation level of group M1 and M2 was decreased significantly ( P < 0. 01) , however, the apoptosis rate of cells was increased significantly ( P <0. 01). Conclusion; Morphine could inhibit the proliferation of the cells and accelerate the cell apoptosis through down-regulating the expression of survivin and livin genes in human breast cancer cell line MCF -7.关键词
吗啡/乳腺癌MCF-7细胞/生存素/livinKey words
morphine/breast cancer cell line MCF-7/survivin/livin分类
医药卫生引用本文复制引用
钱宁,孙灿林,姜琳,赵国军,蒋小芹,于鸿..吗啡对乳腺癌MCF-7细胞生存素及livin表达的影响[J].江苏大学学报（医学版）,2012,22(2):137-140,150,5.基金项目
南通大学自然科学基金资助项目(10Z088) （10Z088）
泰州市311工程人才基金项目(201036) （201036）
