军事医学2012，Vol.36Issue(4)：267-271,275,6.
肿瘤中GLI1RNA编辑的下调及其潜在功能分析
Down-regulation of RNA editing of GLI1 in tumors and analysis of its potential function
摘要
Abstract
Objective To detect the A-to-[ RNA editing in the coding region of cancer associated gene GUI, compare the difference in the editing level between tumor and normal cell lines , and to analyze the potential effect of this editing event in the hope of discovering the role of the RNA editing in the occurrence and progression of cancer . Methods The identification of the A-to-[ RNA editing event and the comparison of the editing level were carried out by comparing the cDNA sequences with their corresponding genomic templates based on PCR , RT-PCR and sequencing methods. The potential functional implications of RNA editing were analyzed by multiple bioinformatics tools . Results An A-to-I RNA editing site mapped to chr 12: 56150891 changed the arginine residue at position 701 of the GUI protein to a glutamine residue, which was detected in the human brain , breast, small intestine tissues and multiple cell lines. Compared with normal controls, the editing level of this site decreased in the hepatoma and breast cancer cell lines . By bioinformatics analysis, this editing event altered amino acid residue and was predicted to destroy putative exonic splicing enhancers ( ESE) and affect putative protein tertiary structure. Conclusion This A-to-I RNA editing event in the coding region of GUI occurs in many diverse human tissues and cell lines. Down^-egulation of RNA editing of GUI in hepatoma and breast cancer cell lines may be associated with the occurrence and progression of cancer .关键词
GLI1/编码区/A-to-I RNA编辑/生物信息学/肿瘤Key words
GUI/ coding region/ A-to-I RNA editing/ bioinformatics/ neoplasms分类
医药卫生引用本文复制引用
张颖,胡亚欧,何涛,侯小强,汪莉,张部昌,王玉民..肿瘤中GLI1RNA编辑的下调及其潜在功能分析[J].军事医学,2012,36(4):267-271,275,6.基金项目
国家自然科学基金资助项目(30800644,31100940) （30800644,31100940）
国家"重大新药创制"科技重大专项资助项目(2012ZX09J12106-03B) （2012ZX09J12106-03B）
