军事医学2012，Vol.36Issue(4)：285-288,4.
利用groupⅡintron构建产气荚膜梭菌突变体
Construction of plc gene of Clostridium perfringens mutant by groupⅡintron
摘要
Abstract
Objective To construct the gene of alpha toxin of Chstridiwn petfringens. Methods pic genes were knocked out without any further modifications by group II intron. Results PCR results showed that the intron RNA had been inserted successfully in the pic genes of the transformants. The colonies did not have any white halo around them on BHI agar containing egg yolk , and their phenotype was a different form of wild type C. Petfringens on Columbia blood agar. Western blotting demonstrated that the pic mutant did not produce alpha toxin protein anymore. Endospore formation and transformability were as stable as those of the wild type. Conclusion The alpha toxin mutant is stable , and can be used as a delivery vehicle to develop mucosal vaccines.关键词
产气荚膜梭菌/α毒素/细菌毒素类/口服疫苗Key words
Clostridiumpetfringens/ alpha toxin/ bacterial toxins / mucosal vaccines分类
医药卫生引用本文复制引用
马臣杰,宋孚洋,高姗,康琳,王玉炯,王景林..利用groupⅡintron构建产气荚膜梭菌突变体[J].军事医学,2012,36(4):285-288,4.基金项目
病原微生物生物安全国家重点实验室开放课题资助项目(PBS2009B-05) （PBS2009B-05）
