江西农业学报2012，Vol.24Issue(1)：109-111,3.
甜蛋白thaumatin基因高效植物表达载体的构建
Construction of High-efficient Plant Expressive Vector for thaumatin Gene
摘要
Abstract
According to the amino acid sequence of the sweet protein gene thaumatin and plant biased codons, the whole gene sequence primer of thaumatin I was designed. The whole gene of thaumatin I was artificially synthesized by overlap extension PCR method , and then it was cloned to pMD\9 - T. The sequence analysis result showed that the synthesized DNA sequence was entirely accordant with the original designed sequence. The high - efficient plant expressive vector pRIlOl - thaumatin was finally constructed by u-sing the expressive vector pRl10l -ON.关键词
时蛋白/thaumatin/pRI101-thaumatin/表达载体Key words
Sweet protein/ thaumatin gene/ pRI1Ol -thaumatin/ Expressive vector分类
农业科技引用本文复制引用
牛苏燕,蒋素华,武思,张国付,崔波,叶永忠..甜蛋白thaumatin基因高效植物表达载体的构建[J].江西农业学报,2012,24(1):109-111,3.基金项目
郑州市科技攻关项目(083STRF40346-2). （083STRF40346-2）
