冯建昆 1韩志华 1吴华 1梁军 1潘玉善 1杜向党 1胡功政1
作者信息
- 1. 河南农业大学牧医工程学院,河南郑州450002
- 折叠
摘要
Abstract
Objective: The incompatible groups of the plasmids carrying tetracycline resistant genes in 22 duck Escherichia coli I-solates were determined by using PCR method. Methods; 18 pairs of designed primers were divided into five multiple PCR systems and three single PCR systems, and they were used to detect FIA, FIB, FIC, H11, H12, II, L/M, N, P, W, T, A/C, K, B/0, X, Y, F and FⅡ replicons. Results; seven, four, nine, two, six, two, three, seven and nineteen of 22 E. Coli isolates from duck belonged to Incll, IncN, IncFIB, IncY, IncP, IncFIC, IncA/C, IncK and IncFH, respectively. Most (86.4%) of plasmids in the tested strains belonged to IncF11. One incompatible group and 2,3,4 and 5 incompatible group combinations accounted for 13. 6% , 31. 8% , 31.8% , 18.2% and 4.5% , respectively.关键词
质粒不相容群/鸭/大肠杆菌/四环素耐药基因/IncFⅡ质粒Key words
Plasmid/ Incompatible group/ Duck/ E. Coli/ Tetracycline resistant gene/ IncFⅡ plasmid分类
农业科技
