江西农业学报2012，Vol.24Issue(3)：11-14,4.
根癌农杆菌介导红颊草莓遗传转化体系的建立
Establishment of Agrobacterium -mediated Transformation System for ‘ Benihope' Strawberry
摘要
Abstract
Taking the leaves of strawberry cultivar ' Benihope' as experimental acceptor, a higher - efficient genetic transformation system was obtained through the optimization of series of influential factors, such as hygromycin selection stress, type and concentration of antibiotics, agrobacterial concentration, infection time and co - culture time. The results showed that when the agrobacterial concentration was OD600 =0.4, the infection time was 6 min, 250 mg/L Cb was used as the antibacterial antibiotics, 5 mg/L hygromycin was used as the screening antibiotics, and the co - culture time was 3 days, this transformation system was highly efficient, and its inducement rate of resistant shoots reached 25% .关键词
遗传转化/草莓/红颊Key words
Genetic transformation/ Strawberry/ Benihope分类
农业科技引用本文复制引用
古咸彬,王翡,高志红,乔玉山,章镇,林之林,糜林,李金凤..根癌农杆菌介导红颊草莓遗传转化体系的建立[J].江西农业学报,2012,24(3):11-14,4.基金项目
江苏省农业资源开发局项目(2008KJ-046) （2008KJ-046）
2011年国家大学生创新训练计划. （）
