军医进修学院学报2011，Vol.32Issue(9)：952-955,4.
以miRNA为基础的RNA干扰线粒体相关基因TFAM和POLG的研究
miRNA-based RNAi mitochondria-related genes TFAM and POLG
摘要
Abstract
Objective To provide the data for mitochondria and stem cells by studying lentivirus -mediated RNAi mitochondria-related genes TFAM and POLG. Methods Mitochondria-related genes TFAM or POLG were selected. Three shRNAs were designed to construct RNAi lentivirus vectors based on miRNA. Lentivirus plasmids were packaged into 293FT cells, transfected into primary fibroblasts, and efficiency of RNAi was detected by quantitative PCR and Western blot, respectively. Results POLG-shRNA-1 decreased the TFAM and POLG expression of 75% and 85%, respectively. Conclusion Lentivirus-mediated RNAi vector can interfere the expression of miRNA-based POLG and TFAM.关键词
RNA干扰/慢病毒/线粒体转录因子A/线粒体DNA特异性聚合酶GKey words
RNA Interference/ Lentivirus/ mtTFA/ POLG分类
医药卫生引用本文复制引用
刘凤斌,李建福,陈晓芳,汤苏阳..以miRNA为基础的RNA干扰线粒体相关基因TFAM和POLG的研究[J].军医进修学院学报,2011,32(9):952-955,4.基金项目
国家自然科学基金项目(30973114)Supported by the National Natural Science Foundation of China(30973114) （30973114）
