昆明医学院学报2011，Vol.32Issue(12)：36-40,5.
PD-L1Ig对大鼠肝移植免疫耐受影响的实验研究
The Study of the Immune Tolerance Induced by PD-L1Ig in Rat Liver Transplantation
摘要
Abstract
Objective To explore the effert of PD-Lllg on the immune ioieranre in rats wild liver transplantation. Methods Twenty ROLT models were established by Kamada two-tuft technique, with inbred male Wistar rats and male SI) rats served as donor and recipient respeclive.lv. The ivcipienls were randonib devided into 2 groups according to different treatments: no treatment A (Wistar->SD n -- 10) and B treated with Pl)-Lllg (Wistar→SD n = 10) after the day of operation, the following day, the lourlh day and sixth day. The clinical characters, the animal survival, mental and appelite were observed. The liver and blood samples were taken form rals after one week for delecting liver function and proportions of CD4~CD25 Treg and observing ihe rejection of liver pathology. Results In the. treatment group, the liver function tesi, including serum alaniue anunotrausferase, aspartate aminotransferase and total bilirnbin, was superior to control group (P < 0.05 ). Pathoiog\ of liver revealed there were a large number of mononuelear cells around the portal tract area in blank control group. The inflammation of PD-Lllg group was relatively less serious, only a little accumulation of mflaimnalorv cells, flic liver rejection of blank control was more serious than PD-Lllg group. Referencing Baff marking, the rejection was moderate andsevere in group of PD-Lllg and blank control, respectively. The group of PD-Lllg showed a lower immune response and lighter rejection of liver than the control group. Flow cytometry analysis of CD4+ CD25+ Treg showed that the proportion of peripheral blood CD4+CD25TYeg cells in PD-Lllg group was significantly higher than control group ( P <0.05). Therefore, PD-Lllg group rats showed better negative regulator activity of immune cell. Conclusions The liver functions of PD-Lllg treated rats are better. A low immune response can be induced in the rats of ROLT by PD-Lllg treatment. The proportion of peripheral blood CD4+CD25Treg increases after liver transplantation in rats, which might be a mechanism of immune tolerance.关键词
PD-L1Ig/大鼠/肝移植/Treg细胞Key words
PD-Lllg/ Rat/ liver transplantation/ Treg分类
基础医学引用本文复制引用
刘晓明,胡明道,樊少敏,陈鹏,CHENPeng..PD-L1Ig对大鼠肝移植免疫耐受影响的实验研究[J].昆明医学院学报,2011,32(12):36-40,5.基金项目
云南省科技厅基金资助项目(2008zc136M) （2008zc136M）
