昆明医学院学报2012，Vol.33Issue(2)：4-8,13,6.
枫叶黄酮抑制脂多糖诱导破骨前体细胞Raw264.7细胞激活的作用
Flavonoid Isolated from the Leaves of Acer Truncatum Bunge Inhibits Lipopolysaccharide-induced Activation of Raw264.7Cells
摘要
Abstract
Objective To investigate the inhibitory effects of flavonoid isolated from the leaves of Acer truncatum Bunge on the expression of pro- inflammatory cytokines in Raw264.7 cells stimulated with lipopolysaccharide (LPS) . Methods Inflammatory cell model was structured by LPS- stimulated Raw264.7 cells, the cells were treated with flavonoid (10 μM, 30 μM and 60 μM) prior to LPS (5 mg/mD exposure and the effects of flavonoid on viability of Raw264.7 cells were measured by MTT assay, Nitric oxide (NO) and prostaglandin E2 (PGE2)were measured by ELISA, the effects on the protein levels of pro- inflammatory enzymes, cyclooxygenase- 2 (COX- 2) and inducible nitric oxide synthase (iNOS) , and pro- inflammatory cytokines, tumor necrosis factor- a (TNF- a ) , and interleukin-1β (IL-1β ), inflammatory signaling proteins nuclear factor- K B (NF- K B) were analysed by immunofl uorescence staining assay. Results LPS-induced NO, PGE2, iNOS, COX-2 and NF- K B protein expression levels were significantly decreased by flavonoid in a concentration- dependent manner and flavonoid also had an effect on the expression of TNF-a , IL-1β through translational inhibition. Conclusion Flavonoid isolated from the leaves of Acer truncatum Bunge can inhibit LPS- induced activation of Raw264.7 cells through inhibiting the release of pro- infl ammatory mediators, then exerts its anti- osteoporosis activities.关键词
枫叶黄酮/脂多糖/炎性因子/Raw264.7细胞/免疫荧光染色Key words
Flavonoid/ Lipopolysaccharide/ Pro- inflammatory cytokines/ Raw264.7 cells/ Immuno uorescence staining分类
生物科学引用本文复制引用
邹智荣,宗一,陆地,孙俊,马云兵,曹西南..枫叶黄酮抑制脂多糖诱导破骨前体细胞Raw264.7细胞激活的作用[J].昆明医学院学报,2012,33(2):4-8,13,6.基金项目
国家自然科学研究基金资助项目(30860336,30560170) （30860336,30560170）
云南省中青年学术和技术带头人后备人才培养项目(2009CI033) （2009CI033）
云南省应用基础研究重点项目(2008CC007) （2008CC007）
昆明医学院研究生创新基金项目(2011J01) （2011J01）
