昆明医学院学报2012，Vol.33Issue(2)：40-44,5.
同型半胱氨酸对小鼠骨骼肌组织PDK1的影响
Effect of Homocysteine on PDK1 in Skeletal Muscle Tissue of Mice
摘要
Abstract
Objective To investigate the expression of 3- phosphoinositide-dependent protein kinase 1 (PDKl) in skeletal muscle tissue of hyherhomocysteinemia animal model, to explore the effect of homocysteine on glucose uptake. Methods Twenty healthy mice, six weeks of age, were randomly divided into control group (n= 10) and high hyperhomocysteinemia group (n=10). Normal food was fed in control group; Normal food and 1.5 % (mass fraction) methionine were fed in hyperhomocysteinemia group. After the feed in the hyperhomocysteinemia group and the control group were fed for three months, the levels of blood glucose were determined in the feeding. The variations of PDK1, phosphorylation of PDK1 and phosphorylation of Akt were observed with Immunohistochemistry in skeletal muscle cells. The expressions of PDK1, phosphorylation of PDK1, Akt and phosphorylation of Akt were determined with Western Blot in skeletal muscle tissue. Results The feeding plasma glucose in the hyperhomocysteinemia group were higher than that in the control group (P< 0.05) . The gray values of PDK1, p- PDK1 (Ser-241) and p-Akt (Thr-308) were increased in the herpyhomocysteinemia group with Immunohistochemistry compared with the control group (P<0.05) . The levels of PDK1, p- PDK1 (Ser-241) and p-Akt (Thr-308) were the lower in the homocysteinemia group with Western Blot (P<0.05). However, the expression of Akt protein was not difference between the homocysteinemia group and the control group (P>0.05). Conclusion Homocysteine may decrease the expression of PDK1 and phosphorylation of PDK1 in skeletal muscle tissue, thus inhibits glucose uptake.关键词
高同型半胱氨酸血症/骨骼肌组织/PDK1/苏氨酸激酶Key words
Hyperhomocysteinemia/ Skeletal muscle tissue/ 3-phosphoinositide-dependent protein kinasel/ Akt分类
医药卫生引用本文复制引用
顾玉,王昕,郭家智,王雅楠,王芳,李树德..同型半胱氨酸对小鼠骨骼肌组织PDK1的影响[J].昆明医学院学报,2012,33(2):40-44,5.基金项目
云南省科技厅一昆明医学院联合专项基金资助项目(2009CD213,2010CD223) （2009CD213,2010CD223）
云南省应用基础研究面上项目(2010CD075,2007Co70M) （2010CD075,2007Co70M）
云南省教育厅基金资助项目(2010C148) （2010C148）
