兰州大学学报（医学版）2012，Vol.38Issue(1)：1-5,5.
缺氧对宫颈癌Hela细胞缺氧诱导因子-1α、葡萄糖转运蛋白-1表达的影响及与细胞增殖的关系
Influence of hypoxia on the expression of HIF-1α and GLUT-1 in cervical cancer Hela cell and its relationship with the cell proliferation in vitro
摘要
Abstract
Objective To investigate the change of expression of hypoxia-inducible factor la (HIF-lα) and glucose transporter-1 (GLUT-1) in cervical cancer Hela cell line under hypoxia environment, and to explore the influence of hypoxia environment upon the expression of HIF-la and GLUT-1 and the cell proliferation in vitro. Methods Hypoxia environment of Hela cell line was induced by CoCl2- MTT assay was used to monitor the cell proliferation in different time and situation, to test the effect of CoCl2-induced hypoxia on the growth activity of human cervicalcancer Hela cell line in vitro. Reverse transcription polymerase chain reaction analysis and im-munocytochemistry were used to examine the changes of HIF-la and GLUT-I in human cervical cancer Hela cell line in vitro under hypoxia in different time. Results The growth activity of human cervical cancer Hela cell line was enhanced when cells were cultured in 50 /imol/L and 100 /xmol/L CoC12 for 24 hours (P <0.05). The proliferation was inhibited by higher concentration of CoC12 (P <0.01). The mRNA expression of HIF-laafter hypoxia exposure for 72 h was higher than that of control group (P <0.01). The mRNA expression of GLUT-1 was significantly increased after hypoxia exposure (P <0.05). Immunofluorescence analysis results showed that both the HIF-la protein and the GLUT-1 protein were increased in hypoxia conditions compared with the normoxia control group (P <0.01). Conclusion Acute or mild hypoxia conditions may promote the proliferation of human cervical cancer Hela cell line. Hypoxia conditions can up-regulate the expressions of HIF-la and GLUT-1 in human cervical cancer Hela cell line and lead to hypoxia tolerance of Hela. The fact that the expression of GLUT-1 protein elevated later than HIF-la protein suggests GLUT-1 gene and protein expression are up-regulated by HIF-la protein.关键词
宫颈肿瘤/缺氧/缺氧诱导因子-1/葡萄糖转运蛋白1型Key words
uterine cervical neoplasms/cell hypoxia/hypoxia-inducible factor 1/glucose transporter type 1分类
医药卫生引用本文复制引用
刘梅毅,杨永秀,欧靖华..缺氧对宫颈癌Hela细胞缺氧诱导因子-1α、葡萄糖转运蛋白-1表达的影响及与细胞增殖的关系[J].兰州大学学报（医学版）,2012,38(1):1-5,5.基金项目
兰州大学“中央高校基本科研业务费专项资金”(lzujbky-2010-145) （lzujbky-2010-145）
