临床口腔医学杂志2012，Vol.28Issue(2)：77-80,4.DOI:10.3969/j.issn.1003-1634.2012.02.004
平阳霉素作用静脉畸形内皮细胞后E-selectin表达
Pingyangmycin-regulated expression of E-selectin in human venous malformation endothelial cells.
摘要
Abstract
Objective:To investigate Pingyangmycin (PYM) -regulated expression of E-selectin in human venous malformation endothelial cells (HVMECs) and mechanism of sclerotherapy of venous malformations with PYM. Method: Expression of the adhesion molecule E-selectin was studied in PYM -stimulated HVMECs by the means of cell ELISA and reverse transcription and the polymerase chain reaction (RT-PCR). Result: E-selectin was induced in a time-dependent fashion after stimulation with PYM. The expression of E-selectin on cell membrane was observed after 2 h exposure to PYM. The highest expression of E-selectin was observed at 6 h. E-selectin started to disappear at 8 h. No significant expression of E-selectin was detected after 24 h exposure. RT-PCR revealed the expression of mRNA of E-selectin started at 2 h. Maximal synthetic activity was observed at 6~8 h for E-selectin. Thereafter, synthesis slowly decreased. E-selectin was induced in a concentration-dependent fashion after stimulation with PYM. After exposure to 1 (μg / mL PYM, HVMECs showed maximal expression of E-selectin compared to other concentration of PYM. Synthesis activity was greatly restrained at 10 μg / mL or higher concentration. Conclusion: Expression of E-selectin in HVMECs can be induced by lower concentration of PYM in a time- and concentration- dependent fashion, and restrained by high concentration of PYM.关键词
内皮细胞/E-selectin/细胞培养/反转录-聚合酶链反应/平阳霉素Key words
endothelial cell/E-selectin/cell culture/reverse transcription and the polymerase chain reaction (RT-PCR)/pingyangmycin分类
医药卫生引用本文复制引用
贾玉林,赵怡芳,贾俊..平阳霉素作用静脉畸形内皮细胞后E-selectin表达[J].临床口腔医学杂志,2012,28(2):77-80,4.基金项目
湖北省自然科学基金资助项目(No.2010CDB07907) （No.2010CDB07907）
