临床神经病学杂志2012，Vol.25Issue(1)：32-35,4.
不同剂量甲泼尼龙治疗实验性变态反应性脑脊髓炎大鼠的疗效及其与大鼠脊髓糖皮质激素受体亚型表达的关系
Curative effects of methylprednisolone with different dosages on experimental allergic encephalomyelitis rats and its relationship with expression of glucocorticoid receptor subtypes in spinal cord
摘要
Abstract
Objective To investigate the curative effect of methylprednisolone ( MP) with different dosages on experimental allergic encephalomyelitis ( EAE) rats and its relationship with expression of glucocorticoid receptor (GR) subtypes in spinal cord. Methods Twenety-eight healthy female Wistar rats were randomly divided into high-dose MP treatment group ( n = 8 ) , low-dose MP treatment group ( n = 8 ) , EAE model group ( n = 7 ) and normal control group ( n = 5 ). The antigen emulsion which made by spinal cord of guinea pig was inoculated to high-dose and low-dose MP treatment groups and EAE group to make EAE models. After then, the score of neurological impairment in those rats was measured daily. Twelve days after immunization, the rats in high-dose and low-dose MP treatment groups were received intravenous injection of MP 100 mg/(kg ? D) and 25 mg/(kg ? D) , respectively for 3 d, then the dosages were halved for 2 d. EAE model group and normal control group were injected with equivalent volume of normal saline. Immunohistochemistry were used to investigate the expression of Gra and GRp in spinal cord tissue of rats. Results The neurological impairment scores in the two MP treatment groups after treatment were significantly dicreased than before (all P <0. 01) , and significant lower than EAE model group (all P < 0. 001) , but there was no statistic difference between each other. The Gra expression in spinal cord tissue in high-dose and low-dose MP treatment groups were both significantly lower than EAE model group and normal control group ( P < 0. 05 -0.01). There was no significant difference of GRp expression among each groups. Correlation analysis showed that the difference value of neurological impairment score before and after MP treatment was not correlated with the expression of Gra or GRp, but was positive correlated with the ratio of Gro/GRp ( r = 0.550, P = 0.027). Conclusion The curative effect of MP on EAE rats is not related to dosage, but correlated with the ratio of Gra/GRp expression in spinal cord tissue.关键词
甲泼尼龙/实验性变态反应性脑脊髓炎/糖皮质激素受体Key words
methylprednisolone/ experimental allergic encephalomyelitis/ glucocorticoid receptor分类
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洪铭范,危智盛,苏全喜,余青云,彭忠兴,臧林泉,黄叶青..不同剂量甲泼尼龙治疗实验性变态反应性脑脊髓炎大鼠的疗效及其与大鼠脊髓糖皮质激素受体亚型表达的关系[J].临床神经病学杂志,2012,25(1):32-35,4.基金项目
广东省自然科学基金(06022782) （06022782）
