临床与实验病理学杂志2012，Vol.28Issue(5)：539-542,4.
荧光原位杂交技术检测肺癌患者痰液中部分染色体数量的改变
Detection of chromosome number abnormalities by fluorescence in situ hybridization in sputum samples from patients with lung cancer
摘要
Abstract
Purpose To study the molecular cytogenetic alterations of the lung cancer with exfoliated cells in sputum samples by Fluorescence in situ Hybridization ( FISH ) analysis of chromosome-specific probes, and evaluate the possibility and validity of FISH in detecting of the lung cancer. Methods FISH was performed using 3,7, 17 and pl6, p53 of chromosome-specific probes to examine chromosome aberration of exfoliated cells in 40 sputum samples from patients suspected of having lung cancer. Results The frequency of numerical aberration of chromosomes 3,7, 17 and pl6, p53 was 41. 9% , 45. 2% , 41. 9% , 54. 9% and 51. 6% in sputum exfoliated cells. Loss of chromosome p16, p53 was the most common finding. As using the 5 chromosome probes in combination for detecting lung cancer, the sensitivity was 80. 6% , the specificity was 77. 8% , diagnostic concordance rate was 80. 0% . Conclusions A number of chromosome aberrations are detected in the lung cancer by FISH technique which provides a basis for further understanding of its molecular pathogenesis, and can be used as an adjunctive tool for the diagnosis, prognosis of lung cancer.关键词
肺肿瘤/荧光原位杂交/痰脱落细胞Key words
lung neoplasms/ fluorescence in situ hybridization/ exfoliated cell分类
医药卫生引用本文复制引用
王飞,章涤,张园,黄艳春..荧光原位杂交技术检测肺癌患者痰液中部分染色体数量的改变[J].临床与实验病理学杂志,2012,28(5):539-542,4.基金项目
新疆维吾尔自治区教育厅青年教师科研培养基金(XJEDU2009S54) （XJEDU2009S54）
