中国临床保健杂志2012，Vol.15Issue(1)：47-49,3.DOI:10.3969/J.issn.1672-6790.2012.01.017
电压依赖性钾通道和钙激活钾通道在高血压大鼠外周血淋巴细胞的变化
Lymphocyte Kv1.3 and KCa3.1 are upregulated in sponstaneously hypertensive rat
摘要
Abstract
Objective To determine express of Kvl. 3,KCa3. 1 mRNA in lymphocyte derived from sponsta-neously hypertensive rat (SHR) and identify the relationship between lymphocyte Kvl. 3,KCa3. Land hypertension. Methods Male SHRs and normotensive Wistar rats were obtained from Experimental Animal Company of Beijing Vital River and Experimental Animal Center of Xinjiang Medical University. Peripheral blood were collected from SHRs and normotensive Wistar rats. Peripheral lymphocytes were separated from heparinized whole blood by standard Ficoll-Hypaque density gradient centrifugation. Real-time PCR was used to detect the expression of Kvl. 3,KCa3. 1 mRNA in lymphocytes. Results ( 1) The expression levels of Kv 1.3 mRNA in lymphocytes from SHRs were significantly increased compared with Wistar rats (P<0.05). (2)Compared with the controls, Kca 3. 1 mRNA were incresed in in lymphocytes from SHRs (P<0.05). (3)Linear correlation existed between systolic blood pressure and expression of Kvl. 3,Kca3.1 mRNA in lymphocytes from SHRs ( r - 0. 830,P = 0.003;r = 0.674,P = 0.03,respectively). Conclusion The lymphocyte Kvl. 3 ,KCa3. 1 mRNA expression are upregulated in SHR,which suggests that Kvl. 3 and Kca 3. 1 maybe contribute to the development of hypertension.关键词
高血压/Kv1.3钾通道/Shaw钾通道/淋巴细胞/大鼠/近交SHRKey words
Hypertension/ Kvl. 3 potassium channel/ Shaw potassium channels/ Lymphocytes/ Rats, inbred SHR分类
医药卫生引用本文复制引用
王凌鹏,罗健,梁平,张源明..电压依赖性钾通道和钙激活钾通道在高血压大鼠外周血淋巴细胞的变化[J].中国临床保健杂志,2012,15(1):47-49,3.基金项目
新疆医科大学第一附属医院青年基金项目 （）
