环境与职业医学2012，Vol.29Issue(2)：77-82,6.
甲状腺癌患者血DNA甲基化分子标志物
Blood DNA Methylation Markers for Thyroid Cancer
摘要
Abstract
[Objective] To identify DNA methylation markers in blood samples of thyroid cancer patients. [Methods] Blood samples from 14 thyroid cancer patients and 10 healthy volunteers were collected in Shanghai. Genomic DNA samples were detected with Illumina Infinium Human Methylation 27K BeadChip for thyroid cancer blood DNA methylation profiles. Fisher, Pearson and Wilcoxon tests were used in statistical and bioinformatic analysis. [ Results ] In comparison of methylation between the case and control groups, 40 differentially methylated genes (29 high methylated genes and 11 low methylated genes) were identified if the statistic threshold was set to P ≤ 5 × 10-4; however, 8 differentially methylated genes (7 high methylated genes and 1 low methylated gene) were identified if P≤1 × 10-4. The latter suggested better ability to diagnose thyroid cancer with the average accuracy of 91.67% in the thyroid cancer diagnosis model. [ Conclusion ] Aberrant methylated genes play an important role in tumorigenesis and could act as molecular markers to diagnosis thyroid cancer in blood.关键词
表观遗传学/甲状腺癌/甲基化芯片/DNA甲基化/分子标志物Key words
epigenetics/thyroid cancer/methylation beadchip/DNA methylation/biomarker分类
医药卫生引用本文复制引用
廖萍,王文静,刘茶珍,罗全勇,王金普,朱佩云,翁康生,顾国浩,蒋敏,吴凡..甲状腺癌患者血DNA甲基化分子标志物[J].环境与职业医学,2012,29(2):77-82,6.基金项目
上海市卫生系统优秀学科带头人计划资助(编号:XBR2011051) （编号:XBR2011051）
上海市科委基础研究重大项目资助(编号:11DJ1400202) （编号:11DJ1400202）
