环境与职业医学2012，Vol.29Issue(2)：86-88,3.
灰霾天气细颗粒物对人支气管上皮细胞凋亡的影响
Effects of Haze Fine Particulate Matter on Apoptosis of Human Bronchial Epithelial Cells
摘要
Abstract
[Objective ] To explore the effect of haze particulate matter PM2.5 on apoptosis of human bronchial epithelial cells (16-HBE). [ Methods ] Groups of cultured 16-HBE cells were exposed to 8,16, 32,64 and 128 μg/mL PM2.5 respectively for 24,48 and 72 h of incubation to detect the cell survival and apoptosis rates. [ Results ] After 24, 48 and 72 h of interference, compared with the negative control group, the cell survival rates of 64 and 128μg/mL groups decreased significantly (P<0.05), and the cell total apoptosis rates increased significantly (P<0.05). Compared with the corresponding 24 h groups, the cell survival rates of 72 h groups decreased significantly (P<0.05), and the apoptosis rates increased significantly except when treated with 8 μg/mL (P<0.05). [ Conclusion ] Haze particulate matter PM2.5 possess cytotoxic effect on 16-HBE to induce apoptosis in a dose- and time-dependent manner.关键词
灰霾天气/细颗粒物/人支气管上皮细胞/细胞凋亡Key words
haze weather/fine particulate matter/human bronchial epithelial cell/cell apoptosis分类
医药卫生引用本文复制引用
覃辉艳,彭晓武,蒙智娟,黄炯丽,李琴,韦小敏,邹云锋..灰霾天气细颗粒物对人支气管上皮细胞凋亡的影响[J].环境与职业医学,2012,29(2):86-88,3.基金项目
2007年国家环保公益性行业科研专项项目(编号:200709004) （编号:200709004）
