棉花学报2012，Vol.24Issue(1)：71-77,7.
棉花内生拮抗菌Paenibacillus xylanilyticus YUPP-1抗黄萎病研究
Biological Control of Cotton Verticillium Wilt by Endophytic Paenibacillus xylanilyticus YUPP-1
摘要
Abstract
To control cotton wilt caused by Verticillium dahliae Kleb, we collected endophytic antagonistic microorganisms during different growth stages and assayed the combined effects. One strain with strong activity against V. Dahliae was isolated from cotton seedlings. This strain was preliminarily identified as Paenibacillus xylanilyticus YUPP-1 by 16S rDNA sequence analysis. This strain was able to successfully colonize cotton roots when it was at concentrations of more than l×l06 cfii per cotton seedling. A pot experiment of the effect of Paenibacillus xylanilyticus YUPP-1 on cotton Verticillium wilt showed the following: in comparison with a control with an infection rate of 96%, only 8%, 5%, and 2% infection rate was observed for the treatments with strain YUPP-1, 1 × 106, 1 × 107, and 1×105 cfii per cotton seedling, respectively. Field tests indicated that control of this disease could be achieved when strain YUPP-1 reached 1 x 10* cfu per cotton seedling. We have thus demonstrated that P. Xylanilyticus YUPP-1 is an effective endophytic biocontrol agent for Verticillium wilt.关键词
棉花/黄萎病菌/解木聚糖类芽孢杆菌/定殖/防效Key words
cotton/ Verticillium dahliae/ Paenibacillus xylanilyticus/ colonization/ disease control分类
农业科技引用本文复制引用
周燚,杨廷宪,王斌先,孙正祥..棉花内生拮抗菌Paenibacillus xylanilyticus YUPP-1抗黄萎病研究[J].棉花学报,2012,24(1):71-77,7.基金项目
农业部"公益性行业(农业)科研专项-棉花黄萎病的综合防治"(nyhyzx07-052) （农业）
农业微生物国家重点试验开放课题"解木聚糖类芽孢杆菌中环糊精糖基转移酶基因的克隆与表达"(AML200806) （AML200806）
湖北省教育厅重大项目"抗棉花枯黄萎病生防菌剂'克萎菌'的研发"(Z20091201) （Z20091201）
国家大学生创新性实验计划"抗棉花枯黄萎病生防菌的筛选"(091048922) （091048922）
