生态与农村环境学报2012，Vol.28Issue(1)：54-60,7.
硫丹对草鱼Ⅰ相、Ⅱ相酶活性及DNA损伤的影响
Effects of Endosulfan on Phase Ⅰ and Ⅱ Enzyme Activities and DNA Damage of Ctenopharyngodon idellus
摘要
Abstract
Effects of endosulfan were investigated on activities of Phase I enzymes (APND, ERND) and Phase II (GST) enzymes in the liver of Ctenopharyngodon idellus and on its DNA were.investigated. The experiment was designed to have four treatments, that is, CK and three levels of endosulfan concentration ( A: 0. 18 , B: 0. 36 and C: 0. 71 μg ? L-1 ). Samples of the fish were taken for analysis separately after they lived in these treatments for 24, 72, 120 and 168 h. Results show that in Treatments B and C the activity of APND was significantly higher after 24 h( P＜0. 05 or P＜0. 01) , but inhibited significantly after 72 h(P＜0. 01) as compared to CK. It was inhibited to a significant extent after 120 h in all the treatments(P＜0. 05 or P＜0. 01). On the whole the activity of ERND was induced by the exposure, whereas the activity of GST was induced at the initial stage and then turned to be inhibited. In Treatments B and C the activity of GST peaked after 72 h of exposure and afterwards declined gradually with the duration of exposure, till 168 h when it turned to be inhibited, while in Treatment A, it peaked after 120 h and declined afterwards, too, till 168 h when it turned off to be equal to that in CK. The damage the exposure of the fish to endosulfan brought to DNA of its liver cells was significant. TL and % TDNA as indicators of DNA damage increased with the increasing dosage of endosulfan in treatment or the extending duration of exposure, showing a significant relationship. In conclusion, endosulfan affects activities of Phase I and Phase II enzymes in the liver of Ctenopharyngodon idellus and causes genetic damage to the DNA of liver cells of the fish.关键词
硫丹/草鱼/酶活性/Ⅰ相酶/Ⅱ相酶/DNA损伤Key words
endosulfan/ Ctenopharyngodon idellus/ enzyme activity/ Phaselenzyme/ Phase II enzyme/ DNA damage分类
资源环境引用本文复制引用
武焕阳,靳涛,许莉佳,Oscar Ortegon,胡颖雄,丁诗华,李云..硫丹对草鱼Ⅰ相、Ⅱ相酶活性及DNA损伤的影响[J].生态与农村环境学报,2012,28(1):54-60,7.基金项目
国家自然科学基金(30670226) （30670226）
重庆市自然科学基金(CSTC,2007BB1373) （CSTC,2007BB1373）
