南京工业大学学报（自然科学版）2012，Vol.34Issue(1)：10-14,5.DOI:10.3969/j.issn.1671-7627.2012.01.003
氧化葡萄糖酸杆菌驯化选育及其产二羟丙酮
Taming and breeding of Gluconobater oxydans and optimum conditions for production of dihydroxyacetone
摘要
Abstract
The glycerol tolerance of the Gluconobater oxydans was improved by adding glycerol to the gradiently seed medium, and the dihydroxyacetone yield was about 29% higher than before taminged. The fermentation conditions were further optimized with one factor experimental design. The effects of glycerol concentration, the amount of yeast extract, CaCO3 concentration and the inoculum size on the fermentation were studied. The results showed that the optimal conditions were as follows:glycerol of 60 g/L,yeast extract of 5 g/L,CaCO3 of 10 g/L,and inoculum size of 4% . Under the conditions,the transformation rate of glycerol was 96% and the dihydroxyacetone yield reached 57. 4 g/L in shaker flask for fermenting 72 h. The transformation rate of glycerol reached 97% and the dihydroxyacetone yield 58. 2 g/L after being cultivated in 7. 5 L fermentor for fermenting 54 h.关键词
甘油/氧化葡萄糖酸杆菌/二羟丙酮/驯化/发酵Key words
glycerol/ duconobacter oxydans/ dihydroxyacetone/ taming/ fermentation分类
化学化工引用本文复制引用
游庆红,戴小燕,朱宏阳,徐虹..氧化葡萄糖酸杆菌驯化选育及其产二羟丙酮[J].南京工业大学学报（自然科学版）,2012,34(1):10-14,5.基金项目
国家自然科学基金资助项目(20906050) （20906050）
江苏省高校自然科学重大基础研究资助项目(08KJA180001) （08KJA180001）
江苏省博士生自然科学类科研创新计划(CX09B_143Z) （CX09B_143Z）
江苏省博士后基金资助项目(0901012B) （0901012B）
