南京农业大学学报2012，Vol.35Issue(1)：14-20,7.
不结球白菜雌蕊蛋白质双向电泳技术体系的建立
Establishment of two-dimensional gel electrophoresis system for protemics analysis of pistils in non-heading Chinese cabbage
摘要
Abstract
To establish a proper two-dimensional gel electrophoresis(2-DE) system for pistils of Brassica campestris ssp. Chinensis Makino, several protein extraction methods, isoelectric focusing procedures and silver staining methods were compared. Consequently, the total proteins were extracted from non-heading Chinese cabbage'Aijiaohuang'pistils by the tris(hydroxymethyl)aminomethane-hy drochloric acid(Tris-HCl)extraction method. The proteins were separated by a improved isoelectric focusing procedure,with 17 cm,pH 4-7 IPG strips and the 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE). The 2-DE map was dyed by the improved silver staining method. The different protein expression by the previous established protocol between bud pistils 2 days before florescence and blossom pistils 2 days after florescence was compared,and 187 distinct protein spots were detected.关键词
不结球白菜/雌蕊/蛋白质/双向电泳Key words
Brassica campestris ssp. Chinensis Makino/pistils/protein/two-dimensional gel electrophoresis分类
农业科技引用本文复制引用
王立,张硕,侯喜林,李英..不结球白菜雌蕊蛋白质双向电泳技术体系的建立[J].南京农业大学学报,2012,35(1):14-20,7.基金项目
国家973计划项目(2009CB119001-04) （2009CB119001-04）
教育部新世纪优秀人才支持计划项目(NCET-07-0439) （NCET-07-0439）
