南京农业大学学报2012，Vol.35Issue(1)：87-91,5.
猪源Mx1基因的原核表达及其抗血清制备
Prokaryotic expression and identification of porcine Mx1 gene and preparation of the mouse antiserum
摘要
Abstract
Based on a pair of specific primers, the open reading frame of porcine Mxl (1 992 bp) gene (poMxl) was amplified from pT-poMxl by PCR. The poMxl gene was cloned into the prokaryotic expression vector of pGEX-6p-l and verified by enzyme digestion and DNA sequencing. Then this recombinant plasmid was transformed into Escherichia coli BL21 (DE3) for overexpression under opit imization conditions. The expression product had a molecular mass about 99×103 as expected. The poMxl protein was separated in gel slices and used to immunize Balb/c mice. Antiserum was prepared and specificity analyzed by Western blot. High titer of antiserum against porcine Mxl protein was obtained. The successful expression of porcine Mxl protein in E. Coli BL21 and the preparation of poMxl specific mouse antiserum laid foundation for the further study on the antiviral mechanism.关键词
猪源Mx1基因/原核表达/抗血清Key words
poMxl gene/ prokaryotic expression/ antiserum分类
农业科技引用本文复制引用
何丹妮,周斌,张小敏,陈溥言,庞然,赵津..猪源Mx1基因的原核表达及其抗血清制备[J].南京农业大学学报,2012,35(1):87-91,5.基金项目
国家自然科学基金青年基金项目(31001062) （31001062）
江苏高校优势学科建设工程资助项目 （）
