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固液气分流式瘤胃模拟系统的设计与测试

沈维军 姜雅慧 王加启 卜登攀 孙鹏 金恩望 包万华 史浩亭

农业工程学报2012,Vol.28Issue(3):20-26,7.
农业工程学报2012,Vol.28Issue(3):20-26,7.DOI:10.3969/j.issn.1002-6819.2012.03.004

固液气分流式瘤胃模拟系统的设计与测试

Design and testing of rumen simulation system with discharging solid chime, liquid, and gas respectively

沈维军 1姜雅慧 2王加启 2卜登攀 3孙鹏 2金恩望 2包万华 2史浩亭2

作者信息

  • 1. 湖南农业大学动物科学技术学院,长沙410128
  • 2. 中国农业科学院北京畜牧兽医研究所,北京100193
  • 3. 中国农业大学生物技术学院,北京100193
  • 折叠

摘要

Abstract

To overcome the disadvantages of the present rumen simulated apparatus, a novel auto-controlled and continuous cultural rumen simulate system was designed, in which solid chime, liquid and gas were constructed in sealed condition. After 10 days testing experiments, the results showed that pH values in all 4 fermenters were stable and consistent with the condition that of in vivo from 5 d to the end of the experiment; protozoal counts kept at (0.99±0.32)×104 (p=0.6877) and the concentration of NH3-N in the overflowed liquid maintained stably at (3.91±1.10) mg/100mL; the rate of total dry matter disappearance, the total times of venting gas and the concentration of total volatile fatty acids were 62%±2%, (2 370±33) and (41.77±3.78) mmol/L (p=0.488), respectively. So, this novel rumen simulate system showed high similarity to bovine rumen fermentation in vivo according to the above parameters and can used to mimic the rumen fermentation in vitro.

关键词

设备/设计/测试/批次培养/连续培养/瘤胃

Key words

equipments/design/measurements/batch culture/continuous culture/rumen

分类

农业科技

引用本文复制引用

沈维军,姜雅慧,王加启,卜登攀,孙鹏,金恩望,包万华,史浩亭..固液气分流式瘤胃模拟系统的设计与测试[J].农业工程学报,2012,28(3):20-26,7.

基金项目

"973"计划(2011CB100805) (2011CB100805)

农业工程学报

OA北大核心CSCDCSTPCD

1002-6819

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