农业环境科学学报2012，Vol.31Issue(2)：351-356,6.
普施特降解菌Bacillus sp.zx2和zx7生长及降解特性
Growth and Degradation Characteristics of Imazethapyr- degrading Bacillus sp.zx2 and zx7
摘要
Abstract
Bacillus sp. Zx2 and zx7 are two effective degradation strains for imazethapyr. Study of their growth and degradation characteristics will provide a scientific basis for the practical bioremediation of soil contaminated by imazethapyer, reduce the harmful effect of imazethapyer on environment and human health and contribute to agricultural sustainable development. Bottle culture method was applied to research the growth characteristics of strains zx2, zx7 and their degradation characteristics for imazethapyr with single and mixed strains. The results showed that both of zx2 and zx7 could grow well in inorganic salt medium when the initial concentration of imazethapyr ≤200 mg·L-1. Strain zx2 showed good growth at the condition: Temperature of 25~35℃ and pH of 4.0~7.0, while the suitable condition for zx7 was temperature of 30～35℃ and pH of 5.0～8.0. Adaptation of the two strains was complementary to each other. Under the optimal growth conditions (temperature 32℃, pH 6.0, and initial imazethapyr concentration of 200 mg?L-1), degradation of imazethapyr by zx2 and zx7 in inorganic salt medium was conformed to the retardation kinetic model. Half-life of imazethapyr degradation was 3.8 days and 2.8 days respectively, and the degradation rates were 85.81% and 90.27% after 6 days culture. In the process of cultivation, pH of zx2 decreased, while pH of zx7 unchanged. It preliminarily showed that the degradation mechanism of zx2 was different from that of zx7. Degradation rate of the mixed strains of zx2 and zx7 was 82.7% which was lower than that of the single strain. It may be due to the reason that zx2 or zx7 utilized the degradation products of each other during their degradating imazethapyr process.关键词
普施特/降解/生长/芽孢杆菌Key words
imazethapyr/ degradation/ growth/ Bacillus sp.分类
资源环境引用本文复制引用
王磊,李淑芹,郑玉莲,许景钢..普施特降解菌Bacillus sp.zx2和zx7生长及降解特性[J].农业环境科学学报,2012,31(2):351-356,6.基金项目
东北农业大学创新团队基金(CXT003-1-3) （CXT003-1-3）
黑龙江省博士后基金项目(LBH-206162) （LBH-206162）
