农业环境科学学报2012，Vol.31Issue(3)：491-497,7.
O3浓度升高对麦田土壤氨氧化细菌、氨氧化古菌和硝化细菌数量的影响
Effects of Elevated Ozone on Quantity of Ammonium-oxidizing Bacteria, Ammonia-oxidizing Achaea and Nitrobacteria in Wheat Field Soil
摘要
Abstract
Nitrification is of vital importance in nitrogen cycle, including ammoxidation and nitration processes. The nitrogen is transformed into NO3 form which is available for the plants through the ammoxidation and nitration. The study aimed at the impact of near the surface O3 concentration on the quantities of ammonium-oxidizing bacteria(AOB), ammonium-oxidizing achaea(AOA) and nitrobacteria(NOB) by the real-time polymerase chain reaction(PCR), open-the top chambers(OTCs) and simulated-smoke experiment in the field. The results showed that the responses of AOB, AOA and NOB under the stress of O3 were different. The quantity of AOB gene copies had a trend of a decrease with the increase of O3 concentration, but the quantities of AOA and NOB gene copies were not changed significantly with the increase of O3 concentration. In the jointing stage of winter wheat, when O3 concentration were 40, 80 and 120 nmol ·mol-1, the quantities of AOB gene copies in 20-40 cm soil were respectively lower than the control group by 39.8%, 51.2% and 59.4%. The quantities of AOB and NOB gene copies in filling stage were more than those in the harvest time, and the quantities of AOB and NOB gene copies in 0~10 cm soil layer were more than those in 10~20 cm soil layer. The quantity of AOA gene copies had not changed significantly with the change of season. In summary, changes in the quantities and activities of AOB, AOA, NOB in response to different O3 concentrations provide a good indicator for investigating the nitrification, which can be used as indicator for the nitrogen cycling in soil.关键词
O3浓度升高/氨氧化细菌/氨氧化古菌/硝化细菌/定量PCRKey words
elevated O3 concentration/ ammonium-oxidizing bacteria/ ammonium-oxidizing achaea/ nitrobacteria/ real-time polymerase chain reaction分类
资源环境引用本文复制引用
伍文,黄益宗,李明顺,于方明,钟敏,隋立华,郝晓伟..O3浓度升高对麦田土壤氨氧化细菌、氨氧化古菌和硝化细菌数量的影响[J].农业环境科学学报,2012,31(3):491-497,7.基金项目
国家自然科学基金面上项目(41071336) （41071336）
国家环保公益性行业科研专项(200809152) （200809152）
