农药学学报2012，Vol.14Issue(1)：35-41,7.DOI:10.3969/j.issn.1008-7303.2012.01.05
连续施药及室内药剂驯化对番茄灰葡萄孢对咯菌腈敏感性的影响
Effect of continuous application and taming in vitro on sensitivity of Botrytis cinerea in tomato to fludioxonil
摘要
Abstract
To clarify the effect of continuous spraying of fludioxonil on sensitivity of Botrytis cinerea in tomato to fludioxonil, a fungicide spraying programming was conducted in selected tomato greenhouse where fludioxonil was sprayed at the recommended rate(active ingredients 67.5 g/hm2 ,a. I. ) for 7 times regularly since the initial occurrence of gray mould, the sensitivity of B. Cinerea to fludioxonil was detected through mycelial growth rate test. The results showed that the B. Cinerea was still sensitive to fludioxonil after 7 continuous sprays, with EC50 values ranging from 0. 004 7 to 0.046 2μg/mL. Two mutant strains (Rg-12 and Rdz-28) resistant to fludioxonil were selected fromthirty sensitive strains of B. Cinerea which were subcultured for 7 generations on the PDA medium that contained with fludioxonil. The EC50 value of Rg-12 to fludioxonil was 46 times of its parent strain g-12 and the EC50 value of Rdz-28 to fludionxonil was more than 500μg/mL. The mycelial growth rate, sporulation and sclerotia of the two mutants decreased obviously, comparing with their parent strains. All the results showed that the level resistance of B. Cinerea to fludioxonil is low, then fludioxonil could be used to control B. Cinerea as an ideal fungicide.关键词
灰葡萄孢/咯菌腈/连续施药/药剂驯化/敏感性Key words
Botrytis cinerea/fludioxonil/continuous application/fungicide taming/sensitivity分类
农业科技引用本文复制引用
纪军建,王文桥,张小风,赵建江,韩秀英,马志强,张金林..连续施药及室内药剂驯化对番茄灰葡萄孢对咯菌腈敏感性的影响[J].农药学学报,2012,14(1):35-41,7.基金项目
农业公益性行业科研专项(200903033). （200903033）
