农药学学报2012，Vol.14Issue(1)：61-66,6.DOI:10.3969/j.issn.1008-7303.2012.01.09
超高效液相色谱-串联四级杆质谱联用法测定水果中多效唑、氯吡脲和咪鲜胺的残留
UPLC-MS/MS method for determination of paclobutrazol,forchlorfenuron and prochloraz residues in fruits
徐永 1寿林飞 1虞淼 1徐正宁2
作者信息
- 1. 浙江省农药检定管理所,杭州3 10020
- 2. 南京大学大气科学学院,南京210032
- 折叠
摘要
Abstract
A rapid method for determination of paclobutrazol, forchlorfenuron and prochloraz residues in watermelon and waxberry was established using ultra high-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). The sample was extracted with acetonitrile and cleaned by solid phase extraction ( SPE) cartridge. The mass spectrometer was operated with electrospray in the positive ion mode (ESI+). The target analytes were quantified by external standard method and matrix-matched standard solution used to compensate for matrix effect. Average recoveries of paclobutrazol,forchlorfenuron and prochloraz in watermelon samples were in the range of 85.4% -99.4% ,85.6% -98.7% and 88. 8% - 100. 3% at three spiking levels from 0. 05 -0. 2 mg/kg with relative standard deviations of 2.7% -4.0%,2.5% -3.6% and 2. 3% -4.6%. Average recoveries of paclobutrazol,forchlorfenuron and prochloraz in waxberry samples were found in the range of 84. 3%-99. 3% ,84. 6% -98. 2% and 83.6% -99.2% at three spiking levels from 0.05 -0.2 mg/kg with relative standard deviations of 2.4% -4.5% ,2.9% -3.7% and 2.2% -3.9%. Limits of detections (LOD) of paclobutrazol, forchlorfenuron and prochloraz in fruits were 0.5,0.5 and 0.5μg/kg, while limits of quantifications (LOQ) were 1.7,1.7 and 1.7μg/kg. The method is simple and suitable for the routine and confirmation analysis on fruits.关键词
超高效液相色谱-串联四级杆质谱/水果/多效唑/氯吡脲/咪鲜胺/残留Key words
ultra high-performance liquid chromatography-tandem mass spectrometry/fruits/paclobutrazol/forchlorfenuron/prochloraz/residue分类
农业科技引用本文复制引用
徐永,寿林飞,虞淼,徐正宁..超高效液相色谱-串联四级杆质谱联用法测定水果中多效唑、氯吡脲和咪鲜胺的残留[J].农药学学报,2012,14(1):61-66,6.
