中国海洋大学学报（自然科学版）2012，Vol.42Issue(1)：54-58,5.
凡纳滨对虾翻译控制肿瘤蛋白Fortilin的毕赤酵母表达及其对血细胞免疫反应的影响
Secretion Expression of Fortilin in Yeast Pichia pastoris and Its Effects on Immune Responses of Hemocytes in White Shrimp Litopenaeus vannamei
摘要
Abstract
Fortilin is a multifunctional protein involved in important cellular activities. In addition, shrimp fortilin may interfere with viral infection, probably by inhibiting viral replication. The fortilin coding sequence (GenBank ID: DQ231062) was amplified by PCR from shrimp (Litopenaeus vannamei') hepatopancreas cDNA. The purified PCR fragments were cloned into the EcoR I/Xba I sites of the pGAPZaA vector. Sequences analysis verified whether the target genes were correctly inserted in right reading frame. The construct was linearized and was integrated into the yeast Pichia pastoris X-33 by electroporation under the selection of Zeocin. The expressed proteins were identified by SDS-PAGE and characterized by matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDITOF MS). Then the culture supernatants of recombinant yeasts were lyophilized for analysis of immune responses of cultured hemocytes of white shrimp. Results showed that Fortilin fusion proteins significantly improved the activities of phenoloxidase and superoxide dismutase of cultured hemocytes. The present study made the first step for application of Fortilin protein in shrimp feeds.关键词
凡纳滨对虾/fortilin/毕赤酵母/血细胞/免疫Key words
Litopenaeus vannamei/ fortilin/ Pichia pastoris/ hemocytes/ immunity分类
农业科技引用本文复制引用
周怡,张文兵,麦康森..凡纳滨对虾翻译控制肿瘤蛋白Fortilin的毕赤酵母表达及其对血细胞免疫反应的影响[J].中国海洋大学学报（自然科学版）,2012,42(1):54-58,5.基金项目
国家公益性行业(农业)科研专项(201103034)资助 （农业）
