中国海洋大学学报（自然科学版）2012，Vol.42Issue(1)：88-92,5.
单环刺螠纤溶酶UFEⅠ药效作用和免疫原性的初步研究
Pharmacologic Effects and Immunogenicity of Fibrinolytic Enzyme UFE Ⅰ from Urechis unicinctus
摘要
Abstract
To study the anticoagulability and antithrombotic activity, and to evaluate the itnmunogenicity of fibrinolytic enzyme UFE I from Urechis unicinctus, preliminary, the effects of anticoagulability and antithrombotic activity were observed by the the experiments of thrombolysin on coagulation time in mice and carotid thrombosis in rats respectively. The dynamic changes of IgG and the antibody titers were detected by ELISA method after immunizing the mice with UFE I. The results inchicated that UFE I significantly prolonged the coagulation time in mice, and effectively inhibited thrombosis of rats in vivo. Moreover, the fibrinogen content (FIB) was decreased, whereas the activated partial thromboplastin time ( APTT) was prolonged. In case of the injury experiment in mice, the specific antibody appeared after one week, which dynamic tendency of initial rising and later dropping. The specific antibody titer was to be 1; 1280 by the optimal ELISA method. Conclusion was achieved that UFE I possesses significant effects on anticoagulability and antithrombotic activity, which induces a temporary rise of specific antibodies in mice, but does not affect adversely on animal bodies.关键词
单环刺螠/纤溶酶/抗凝血作用/抗血栓作用/免疫原性Key words
Urechis unicinctus/ fibrinolytic enzyme/anticoagulability/antithrombotic activity/ immunogenicity分类
生物科学引用本文复制引用
刘万顺,成慧中,韩宝芹,毕庆庆,董文,关驰..单环刺螠纤溶酶UFEⅠ药效作用和免疫原性的初步研究[J].中国海洋大学学报（自然科学版）,2012,42(1):88-92,5.基金项目
国家"十一五"重大专项(2009ZX09103-646)资助 （2009ZX09103-646）
