中国海洋大学学报（自然科学版）2012，Vol.42Issue(1)：106-112,7.
低分子量海带岩藻聚糖硫酸酯的制备及流感病毒神经氨酸酶抑制活性研究
Preparation and Inhibitory Activity on Influenza Virus Neuraminidase of Low Molecular Weight Fucoidan from Laminaria japonica
摘要
Abstract
Low molecular weight fucoidan was prepared by free radical oxygen degradation and ultrafiltration technology from high molecular weight fucoidan extracted from Laminaria japonica. The molecular weight and chemical composition of low molecular weight fucoidan fractions obtained by different degradation conditions were compared and the optimum degradation condition to yield highly sulfated low molecular weight fucoidan was 4. 5% H2O2 and 0. 0267mol/L Cu2+ from 4. 5 g crude fucoidan. Two highly sulfated fractions Fa3(Mw 7. 68 kDa)and Fb3(Mw 3. 89 kDa)were obtained with the sulfated ester content of 30. 32% and 32. 48% respectively. Low molecular weight fraction Fa3 was mainly made up of fu-cose (64. 25%) and galactose (30. 74%), while the fucose content of fraction Fb3 decreased to 46. 94%, though it had a higher content of sulfated ester. When the concentration of H2 O2 increased to 9 %, the molecular weight and content of sulfated ester and fucose of low molecular weight fucoidan fractions decreased significantly. The antiviral effect on influenza virus of low molecular weight fucoidan was assessed by fluorometric assay of influenza virus neuraminidase in vitro. Low molecular weight fucoidan exhibited significantly inhibitory effect on the activity of neuraminidase in vitro, especially fraction Fa3 with Mw 7. 68 kDa had better inhibitory activity than Fb3 and Fc3. The results revealed that molecular weight played a more important role on determining the inhibitory activity of low molecular weight fucoidan on neuraminidase in vitro than sulfated ester content. The homogeneous neutral sugar composition may also contribute to the inhibitory activity on influenza virus neuraminidase of Fa3.关键词
低分子量岩藻聚糖硫酸酯/自由基氧化降解/神经氨酸酶Key words
low molecular weight fucoidan/radical process degradation/ neuraminidase分类
轻工纺织引用本文复制引用
李芳,李八方,董诗竹,王景峰,赵雪..低分子量海带岩藻聚糖硫酸酯的制备及流感病毒神经氨酸酶抑制活性研究[J].中国海洋大学学报（自然科学版）,2012,42(1):106-112,7.基金项目
国家自然科学基金项目(30800858) （30800858）
山东省自然科学基金项目(ZR2010CQ020)资助 （ZR2010CQ020）
