热带作物学报2011，Vol.32Issue(11)：2029-2036,8.
元素缺乏和外加碳源对小球藻(Chlorella sp KMMCC FC-21)生长和油脂积累的影响
Nutrien Elements Deficiency and Carbon Source Addition Affect on the Growth and Lipid Accumulation in Chlorella sp KMMCC FC-21
摘要
Abstract
A high oil microalgae strain collected from Hainan was defined as Chlorella sp KMMCC FC-21 through 18S rDNA identification in the research. This strain was grown in the high carbon medium HSM, low carbon medium BG11 and SE, and their nitrogen(N), sulfur (S)and phosphorus (P) free medium respectively to study the effects of these three elements deficiency on the biomass and oil accumulation. Results showed that N and S starvation led to dramatically increase in cellular lipid content, especially in high carbon medium, which was accompanied by remarkable decrease of biomass. However, the starvation of P could only give rise to slight increase of lipid content. When cultured in SE-S with 30 mmol/L sodium acetate and 25 mmol/L glucose respectively, the corresponding biomass of Chlorella sp KMMCC FC -21 was increased by 200% and 160% compared to those in SE-S, and the corresponding lipid content was increased by 120% and 72.7%. Moreover, the component of cells was also detected. The results indicated that cells grown in HSM-N, HSM-S and HSM -P contained higher lipid content but lower total protein, total carbohydrate content, chlorophyll content and photosynthesis efficiency comparing to the control.关键词
小球藻/元素缺乏/生物量/油脂积累/碳源/光合效率Key words
Chlorellavidgaris/ Element restriction/ Biomass/ Lipid content/ Carbon source/ Photosynthetic efficiency分类
化学化工引用本文复制引用
蔡佳佳,费小雯,李亚军,胡新文,郭建春,邓晓东..元素缺乏和外加碳源对小球藻(Chlorella sp KMMCC FC-21)生长和油脂积累的影响[J].热带作物学报,2011,32(11):2029-2036,8.基金项目
国家自然科学基金项目(No.30860028、No.31000117) （No.30860028、No.31000117）
中央级公益性科研院所基本科研业务费(No.ITBBZX0941)海南省工程技术研究中心专项(No.GCZX2011006). （No.ITBBZX0941）
