热带作物学报2011，Vol.32Issue(11)：2048-2053,6.
巴西橡胶树HbRPS6-2基因的克隆与生物信息学分析
Cloning and Bioinformatic Analysis of the Gene HbRPS6-2 from Hevea brasiliensis
摘要
Abstract
A high homologous RPS6 gene sequence was isolated by screening Brazilian rubber tree methylation filtration library. According to its sequences information, a novel full-length RPS6 termed HbRPS6-2 was obtained by rapid amplification of cDNA ends (RACE). The sequence contained 1 327 bp in length with a 483 bp open reading frame (ORF)> flanked by a 357 bp 5'-UTR and a 475 bp 3'-UTR. It encoded 161 amino acids. No signal peptide and transmembrane structure were predicted within HbRPS6-2. The result of secondary structure predicted showed that the protein was classified as mixed type. There was a strong hydrophobic region in the N-terminal 18 amino acids of HbRPS6-2, but the entire polypeptide chain was hydrophilic. The result of phylogenetic tree indicated that HbRPS6-2 displayed a high degree of sequence similarity to PaRPS6, and had a low degree of sequence similarity to AtRPS6, ZmRPS6 and so on.关键词
巴西橡胶树/HbRPS6-2/基因克隆/序列分析Key words
Hevea brasiliensis/ HbRPS6-2/ Gene cloning/ Sequence analysis分类
农业科技引用本文复制引用
涂敏,蔡海滨,华玉伟,黄华孙..巴西橡胶树HbRPS6-2基因的克隆与生物信息学分析[J].热带作物学报,2011,32(11):2048-2053,6.基金项目
农业部农作物种质资源保护项目(NB11-2130135-39) （NB11-2130135-39）
中央级公益性科研院所基本科研业务费专项(No.1630022011003). （No.1630022011003）
