热带作物学报2011，Vol.32Issue(11)：2076-2079,4.
甘蔗铁氧还蛋白基因cDNA的克隆及序列分析
Cloning and Sequence Analysis of Ferredoxin cDNA from Sugarcane
摘要
Abstract
Ferredoxin(Fd)cDNA was amplified by RT-PCR from sugarcane leaves, and then it was ligated into pMD18-T vector and the corresponding sequences were analyzed. The results indicated that the fragment length was 688 bp, including Fd cDNA ORF encoding 151 amino acids. Amino acid sequence analysis showed that there were some acidic amino acid residues, and those amino acid sequences between 76 to 101 were highly conserved. Sequences alignment showed that the homology of cDNA sequence between sugarcane and maize Fd gene was 93.68%, while the homology of amino acid between them was 95.39%. Relative protein molecular weight was 16.05 ku, PI was 4.72, prohydrophobicity from-2.567 to 1.344. The molecular structure prediction results suggested that the protein contained 13.9% a-helix, 25.8% p-meander and 60.3% loop. The sequence cloned in this study was named as Fdl, which has been submitted to GenBank with Accession number of GU320210.1. The present study should lay a base for the function study of this gene in sugarcane.关键词
甘蔗/铁氧还蛋白/基因克隆/序列分析Key words
Sugarcane/ Ferredoxin/ Cloning/ Sequence analysis分类
生物科学引用本文复制引用
宋喜梅,黄峰,姚全胜,郑良永,陈由强..甘蔗铁氧还蛋白基因cDNA的克隆及序列分析[J].热带作物学报,2011,32(11):2076-2079,4.基金项目
现代农业产业技术体系建设专项资金 （）
中央级公益性科研院所基本科研业务费专项基金(中国热带农业科学院南亚热带作物研究所)(No.sscri200720). （中国热带农业科学院南亚热带作物研究所）
