热带作物学报2011，Vol.32Issue(12)：2274-2277,4.
AGPase基因转化木薯获得转基因植株的研究
Development of Transgenic Cassava Plants Using AGPase Gene Transformation
摘要
Abstract
AGPase gene functioning to increase starch content under the control of root tuberous specific expression promoter (Sporamin)was successful introduction into cassava SC8 genome by Agrobacterium-mediated transformation. 25 transgenic cassava plants were obtained, and the transformation frequency was 1.20%. 19 of 25 transf(rmed plants can root in rooting resistance medium; PCR and Southern blot analysis showed that AGPase gene was integrated into the genome of 19 transgenic cassava plants.关键词
AGPase基因/木薯/转基因植株/农杆菌介导Key words
AGPase gene/Manihot esculenta Crantz/Transgenic plant/Agrobacterium-medialed分类
农业科技引用本文复制引用
郭运玲,姚庆荣,孔华,刘恩平,贺立卡,郭安平..AGPase基因转化木薯获得转基因植株的研究[J].热带作物学报,2011,32(12):2274-2277,4.基金项目
国家自然科学基金项目(No.30960204),现代农业产业技术体系项目(No.nycytx-17),国家重点基础研究发展计划(973)项目(No.2010CB126605). （No.30960204）
