热带作物学报2011，Vol.32Issue(12)：2324-2330,7.
基于DNA序列的12种粉蚧亲缘关系分析
Genetic Relationship of 12 Species of Mealybugs(Hemiptera:Pseudococcidae)Based on DNA Sequences
摘要
Abstract
The rDNA 18S and 28S of 14 populations of 9 species of mealybug, including Dysmicoccus brevipes, D. Neobrevipes, Planococcus minor, Pseudococcus citri, P. Comstock, P. Baliteus, Saccharicoccus sacchari, Phenacoccus solenopsis, Oracella acuta were sequenced. These sequences were analyzed with ClustalX 2.0, MEGA 4.1 and PAUP 4.10 and so on, and the homological sequences of other 3 species available in GenBank retrieved for DNA alignment and clustering. The feasibility of the application of 18S & 28S sequences to the phylogenetic reconstruction and molecular identification of the 12 species of mealybug was then evaluated. Alignment of sequenced 18S & 28S showed that they were highly conserved within intraspecific taxa but that significant genetic distances existed between interspecific taxa. Comparison and analysis of the genetic distance led to the clear conclusion that 18S & 28S are suitable only for species-level identification. Sequences of rDNA 18S &28S failed to distinguish P. Minor, P. Citri, and P. Ficus from each other for their highly similarity, and the difference of mtDNA COI sequences of them are enough to distinguish each other. The genetic distance between the collections of P. Baliteus from two different hosts was approximately 0.014. These data suggest that P. Baliteus might be a species complex which includes at least two cryptic lineages or sibling species.关键词
粉蚧科/核糖体18S/28S基因/线粒体COI基因/亲缘关系Key words
Pseudococcidae/rDNA 18S&28S/mtDNA COI/Genetic relationship分类
生物科学引用本文复制引用
何衍彪,万宣伍,詹儒林,孙光明,刘映红,许再福,赵艳龙..基于DNA序列的12种粉蚧亲缘关系分析[J].热带作物学报,2011,32(12):2324-2330,7.基金项目
公益性行业科技专项-菠萝现代产业技术体系建立与研究(No.3-41) （No.3-41）
中国热带农业科学院中央级非盈利性研究机构基本科研业务费研究专项(No.sseri200702). （No.sseri200702）
