四川大学学报（自然科学版）2012，Vol.49Issue(1)：193-198,6.DOI:10.3969/j.issn.0490-6756.2012.01.032
草鱼胸腺素β11的cDNA克隆及序列分析
Molecular cloning and characterization of thymosin β11 cDNA in grass carp
摘要
Abstract
The thymosin β11 EST sequence was picked out from the cDNA library of Ctenopharyngodon idellus to design the RACE primers. The full length cDNA sequence of thymosin β11 (GenBank accession number; HM120850) from C. Idellus intestine was cloned by rapid amplification of cDNA ends (RACE) method. The full longth of this gene's cDNA is 320 bp. It has a 47 bp 5'-untranslated region (UTR) and a 141 bp 3'-UTR. The open reading frame (ORF) of this gene's cDNA is 132 bp which putatively encodes 43 amino acids with the isoelectric point of 5. 17 and molecular weight of 4892 Da. Multiple a-lignments showed that the deduced amino acid sequence of β-thymosins from grass crap shares a high degree of identity with β-thymosins sequences from other species, and the highest homology specie is Da-niorerio, (93. 0%). Secondary structure prediction analysis indicated that secondary structure of C. Idellu's thymosin β11 is highly similar with other β-thymosins.关键词
克隆/序列分析/β胸腺素/草鱼(Ctenopharyngodon idellus)Key words
cloning,sequence analysis/β-hymosins/Ctenopharyngodon idellus分类
生物科学引用本文复制引用
辜文博,袁恬,冯军,顾继锐,吴江,刘汉元,徐恒..草鱼胸腺素β11的cDNA克隆及序列分析[J].四川大学学报（自然科学版）,2012,49(1):193-198,6.基金项目
通威股份公司科技发展项目-草鱼基因组计划 （）
四川省科技攻关项目(05NG002-002-4) （05NG002-002-4）
