四川大学学报（自然科学版）2012，Vol.49Issue(1)：205-210,6.DOI:10.3969/j.issn.0490-6756.2012.01.034
辛伐他汀对高胆固醇血症小鼠肝脏基因表达的影响
Effects of simvastatin on the gene expression in liver of hypercholesterolemia mice
摘要
Abstract
Suppression subtractive hybridization(SSH)and targeted display(TD)combined to further identify and validate the differentially expressed genes.Seven genes after simvastatin treatment were upregulated and nineteen were down-regulated.The corresponding genes were found to be involved in transcription,lipid metabolism process,and immune reaction.The transcription of utrophin gene,coding for a cytoskeletal protein and sharing structural and functional homology with dystrophin,was found to be downregulated after simvastatin treatment.Utrophin mRNA levels in the liver were further analyzed using real-time quantitative RT-PCR.Tumor necrosis factor α(TNF-α)was simultaneously examined to get the information of inflammatory cytokines and utrophin.The results show that simvastatin decreases mRNA levels of utrophin and TNF-α in the livers of hypercholesteremic mice.关键词
辛伐他汀/基因差异表达/utrophin/TNF-αKey words
simvastatin/gene expression/utrophin/TNF-α分类
生物科学引用本文复制引用
康耀霞,王文瑞,谭雪梅,段向阳,高巍,侯志萍,张义正..辛伐他汀对高胆固醇血症小鼠肝脏基因表达的影响[J].四川大学学报（自然科学版）,2012,49(1):205-210,6.基金项目
国家科技支撑项目(2007BAD78B03) （2007BAD78B03）
