四川大学学报（自然科学版）2012，Vol.49Issue(1)：211-215,5.DOI:10.3969/j.issn.0490-6756.2012.01.035
荧光淬灭法研究黑豆凝集素Ⅰ微环境与构象的关系
Effects of quenchers on micro-environment and conformation of the lectin Ⅰ from Glycine max var.Seed
摘要
Abstract
The effects of intrinsic fluorescence quenching on GML I were studied using KI, CsCl and ac-rylamide. Native GML I exhibited fluorescence emission maximum λTM, at 339 nm upon excitation at 280 nm, indicating the presence of tryptophan in an relatively hydrophobic region. The results indicated that the mechanisms of fluorescence quenching on the GML I caused by three kinds of quenchers were the dynamic quenching. Fluorescence quenching showed that acrylamide, KI and CsCl were able to quench 100%、81.6% and 52. 8% of the intrinsic fluorescence of GML I respectively, further supporting that tryptophan residues are mainly located on the surface or near surface of the GML I molecular and micro-environment surrounding the Trp residues with more positive charge than negatively charge.关键词
黑豆凝集素Ⅰ/荧光淬灭/微环境Key words
Glycine max var. Lectin I (GML I)/fluorescence quenching/micro-environment分类
生物科学引用本文复制引用
伏秦超,刘超,何铃娟,鲍锦库..荧光淬灭法研究黑豆凝集素Ⅰ微环境与构象的关系[J].四川大学学报（自然科学版）,2012,49(1):211-215,5.基金项目
国家自然科学基金(30670469) （30670469）
