四川大学学报（自然科学版）2012，Vol.49Issue(1)：230-238,9.DOI:10.3969/j.issn.0490-6756.2012.01.038
利用比较基因组学方法预测短小芽孢杆菌转录调控网络
A transcriptional regulatory network of Bacillus pumilus predicted by comparative genomcis methods
摘要
Abstract
To explore the gene transcriptional regulatory relations of Bacillus pumilus, a candidate tran-scriptional regulatory network of B. Pumilus has been predicted by searching the homologous transcription factors (TFs) and the homologous regulated genes from Bacillus subtilis genome. The positional weight matrix(PWM) of transcription factor binding sites(TFBSs) was used to scan the whole B. Pumilus genome in order to verify the candidate transcriptional regulatory networks. Finally, a more complete transcriptional regulatory networks had been constructed by integrating with other B. Pumilus TFs and regulated genes. The network consist of 195 TFs and 1201 regulated genes. The result showed that, in bacteria, with the data set of the closely related model species which has a long history of experimental research, it is feasible to predict the transcriptional regulatory network by using comparative genomics methods. The study provided a feasible approach for exploring gene regulatory networks of the organism which lacks large-scale gene expression microarray data.关键词
比较基因组学/短小芽孢杆菌/枯草芽孢杆菌/转录调控网络Key words
comparative genomics/B. Pumilus/B. Subtilis/gene transcriptional regulatory network分类
生物科学引用本文复制引用
桂俊鸿,李校,赵培虎,刘震,王海燕,张义正..利用比较基因组学方法预测短小芽孢杆菌转录调控网络[J].四川大学学报（自然科学版）,2012,49(1):230-238,9.基金项目
国家自然科学基金(61001149,31070071) （61001149,31070071）
教育部博士点基金(200806101013) （200806101013）
