水产学报2012，Vol.36Issue(1)：25-31,7.DOI:10.3724/SP.J.1231.2012.27521
三疣梭子蟹选育家系微卫星分析
SSR analysis of selected populations of inbred Portunus trituberculatus
摘要
Abstract
The genetic structure of different trituberculatus generations was analyzed based on microsatellite DNA markers. Sixteen microsatellite markers were used to analyze the genetic structure and genetic diversity of the selected populations of inbred Portunus rituberculatus from Fl to F4. The results showed that the genetic diversity of the breeding population was decreasing. The the average polymorphism information content(PIC)of the 16 microsatellite markers decreased from 0.675 3 to 0.406 1 ,the average allele number from 3.5 to 2. 133 3, the average heterozygosity from 0. 643 5 to 0. 477 4 and the average rate of allele homozygous from 0.566 9 to 0. 402 4. We tested all loci for H-W,there are different degrees of deviation from equilibrium in each generation. There are different degrees of genetic differentiation by F-test for each system,and the results show that 19.07% of the genetic differentiation is among populations,80.93% of the genetic differentiation is within the group. In addition, the FIS value calculations show that four families on the whole are expressed as a certain degree of heterozygosity,in which F4 has 12 sites, F3 has six sites,F2 has three sites,F1 ,8 loci in heterozygosity status. Increasing genetic distance,adjacent genetic similarity between generations gradually increased. With the breeding, the results show that after 4 consecutive generations of breeding,the genetic basis of breeding gradually purified and genotype was gradually moving towards homozygous and stable. We expected to get more stable strains with further breeding.关键词
三疣梭子蟹/选育家系/微卫星/遗传多样性Key words
Portunus trituberculatus/ selected populations/ microsatellite marker/ genetic diversity分类
农业科技引用本文复制引用
韩智科,刘萍,李健,高保全,陈萍..三疣梭子蟹选育家系微卫星分析[J].水产学报,2012,36(1):25-31,7.基金项目
国家"八六三"高技术研究发展计划(2012AA10809) （2012AA10809）
国家自然科学基金项目(30871933) （30871933）
农业科技成果转化资金项目(2010GB23260589) （2010GB23260589）
