水产学报2012，Vol.36Issue(1)：50-54,5.DOI:10.3724/SP.J.1231.2012.27540
海带染色体的DAPI染色及核型初步分析
Karyological observation on Saccharina japonica chromosomes stained with DAPI
摘要
Abstract
Saccharina japonica( Aresch. )C. E. Lane,C. Mayes et G. W. Saunders( = Laminaria japonica Aresch. ) (Phaeophyta) is an important economic seaweed in China. There is a distinct outcome about the chromosome number in this kelp due to the tiny size of these chromosomes. The karyotypes and chromosomes of S. Japonica were observed after a series of treatments including pretreatment with 0. 2% colchicines for about 10 h, Carnoy' s fixative, and mixture of enzymes prior to dropping from 30 cm height overhead glass slides for spreading the surface coat. The prepared chromosomes were stained with 4',6-diamidino-2-phenylindole ( DAPI), a fluorescent probe sensitive and specific to DNA. The chromosome numbers of the haploid male and female gametophytes were 31 respectively,and there were 62 in diploid sporophytes. Most of the chromosomes were either droplet or short bacilliform. In the meantime, the female gametophyte chromosomes were between 0. 78 μm and 2. 61 μm in size, larger than the males that were between 0.57 |xm and 2.17 μm. Based upon the relative size of chromosome, the karyotypes of the female or male gametophyte chromosomes were primarily analyzed. All the results laid a solid foundation for a basic technique for localization of molecular markers on kelp chromosomes.关键词
海带/配子体/DAPI/染色体/核型/孢子体Key words
Saccharina japonica/ gametophyte/ DAPI/ chromosome/ karyotype/ sporophyte分类
生物科学引用本文复制引用
刘宇,毕燕会,周志刚..海带染色体的DAPI染色及核型初步分析[J].水产学报,2012,36(1):50-54,5.基金项目
国家自然科学基金项目(30471328,30671627) （30471328,30671627）
国家"八六三"高技术研究发展计划(2012AA100806) （2012AA100806）
上海市自然科学基金项目(10ZR1413900) （10ZR1413900）
上海市教育委员会海洋生物学重点学科资助项目(J50701) （J50701）
