水产学报2012，Vol.36Issue(2)：161-169,9.DOI:10.3724/SP.J.1231.2012.27680
两种杂交石斑鱼子一代杂种优势的微卫星标记分析
SSR analysis of the heterosis in the two grouper hybrids
摘要
Abstract
The marine grouper species are considered high value food fish in Southeast Asia. Aimed to get some new types of grouper, we have successfully cross-bred three species of grouper. The hybrid grouper was produced by fertilising the eggs of the orange-spotted grouper ( Epinephelus coioides) , tiger grouper (Epinephelus lanceolatus) with the sperm of the giant grouper (E. lanceolatus) through the in vitro fertilisation technique. The hybrids combine morphological features of the two parent species. And they are healthy and growing well in sea cages and the finding will be good news for the aquaculture industry. The genetic diversity among orange-spotted grouper, tiger grouper, giant grouper and their hybrid F, generation population(Qinglong grouper and Hulong grouper) were tested by 6 microsatellite loci. The gene frequency, effective number of alleles(Ae), observed heterozygosity (Ho) .polymorphism information contents (PIC), genetic similarity and Nei' s genetic distances(D) were studied. The results showed that,the E. fuscoguttatus showed the most alleles(6.849 3),while the E. lanceolatus showed the least(2.608 6). Rich polymorphisis was observed by the six primers, and the PIC values in the five populations were 0.666 7,0. 751 3,0.441 9, 0.664 0,0. 542 6,respectively. And the F1 generations showed the highest observed heterozygosity(Ho)of 0.940 0,while the E. lanceolatus showed the lowest value of 0. 508 3. The results of genetic distances and genetic similarity showed that both F1 generations had a close relationship with the male parent E. lanceolatus. The alleles of F1 generations were mostly generated from both of their parents, which mean that the genetic materials were inherited from parents. And the higher genetic diversity that existed in the two hybrids indicated potential heterosis.关键词
斜带石斑鱼/鞍带石斑鱼/棕点石斑鱼/杂交子代/微卫星Key words
Epinephelus coioides/ Epinephelus lanceolatus/ Epinephelus fuscoguttatus/ hybrid/ SSR分类
生物科学引用本文复制引用
周翰林,张勇,齐鑫,张海发,张磊,王乐,蒙子宁,刘晓春,林浩然..两种杂交石斑鱼子一代杂种优势的微卫星标记分析[J].水产学报,2012,36(2):161-169,9.基金项目
公益性行业(农业)科研专项(200903046) （农业）
国家自然科学基金面上项目(30871914) （30871914）
广东省科技计划项目(2008B020800010,2010B090400551,2011B020307009) （2008B020800010,2010B090400551,2011B020307009）
广东省海洋渔业科技推广专项(A200800H01,A200900H01) （A200800H01,A200900H01）
