水产学报2012，Vol.36Issue(2)：202-209,8.DOI:10.3724/SP.J.1231.2012.27603
利用微卫星标记对文蛤4个壳色花纹品系的遗传分析
Genetic analysis among four strains of different shell colors and decorative patterns of Meretrix meretrix using microsatellite markers
摘要
Abstract
Genetic variation of four strains of Meretrix meretrix,thin checkered(TC),black spot( BS) ,dark fringe (DF) and red shell (RS) was studied. Thirty randomly selected individuals from each strain and nine polymorphic microsatellite loci were used for genetic analysis. A total of 105 alleles were identified,the most alleles were 21 (WG07) and the least locus were 7 (WG11). The distribution of the alleles for the same loci was different in different strains and specific allele types were detected in each strains. All the microsatellites were highly polymorphic, and various genetic variability measures, including observed heterozygosity(0.486 to 0.867) .expected heterozygosity(0.110 to 1.000) ,and polymorphism information content(0.433 to 0. 834), Chi-square tests showed that most of the cases in four strains deviated from Hardy-Weinberg equilibrium. Pairwise FST ranged from 0.005 to 0.269. Genetic distances among strains ranged from 0.052 0 to 0.098 0. The results of cluster analysis indicated that TC strain had higher genetic difference than other strains,then was BS strain,and the genetic variation between DF and RS strains was relatively lower.关键词
文蛤/壳色花纹/微卫星标记/遗传结构Key words
Meretrix meretrix/ shell color and decorative pattern/ microsatellite markers/ genetic structure分类
生物科学引用本文复制引用
朱东丽,董迎辉,林志华,姚韩韩..利用微卫星标记对文蛤4个壳色花纹品系的遗传分析[J].水产学报,2012,36(2):202-209,8.基金项目
国家自然科学基金项目(30972255,31101895) （30972255,31101895）
浙江省自然科学基金(Y3110080) （Y3110080）
