山东农业科学2012，Vol.44Issue(1)：11-13,3.
转Bt基因玉米叶片中Bt蛋白的表达和分泌研究
Study on Expression and Exudation of Bt Protein in Leaves of Transgenic Insect- resistant Corn
摘要
Abstract
With the transgenic insect - resistant corn Mon810 and its non - transgenic parent as materials , the enzyme - linked immunosorbent assay (ELISA ) method was used to detect the expression and quanti-tate the exudation of Bt protein in corn leaves. The results showed that Bt protein could be detected in tissue and on surface of leaves. The Bt content in tissues showed a rising trend from seedling stage to silking stage with the advances of vegetable growth, and reached the peak at the silking stage and then declined gradually. The variation of Bt exudation on leaf surface had no obvious regularity.关键词
酶联免疫吸附测定法(ELISA)/转基因玉米/Bt蛋白Key words
ELISA/Transgenic corn/Bt protein分类
生物科学引用本文复制引用
田晓燕,赵蕾,孙红炜,李凡,赵辉,颜世磊,路兴波..转Bt基因玉米叶片中Bt蛋白的表达和分泌研究[J].山东农业科学,2012,44(1):11-13,3.基金项目
农业部转基因生物新品种培育重大专项(2009ZX08011 -020B) （2009ZX08011 -020B）
