山东农业科学2012,Vol.44Issue(1):14-16,3.
鲁枣1号组织培养和植株再生体系研究
Study on Tissue Culture and Regeneration System of Jujube Variety Luzao 1
摘要
Abstract
The growing shoots of jujube variety Luzao 1 were used to study the bud induction and rapid propagation of test tube plantlets. The results showed that the latent bud of shoot stem segments could be induced to break on the induction medium, which was MS supplemented with 1 mg/L BA,0. 2 mg/L IB A and 3% sucrose, and the bud break rate was 54. 8%. On the same induction medium, adventitious bud could be induced to regenerate from the distal cut surface of stem section not touch medium, and the regeneration rate was 23. 8% . The propagation of adventitious bud and latent bud was better on the medium of MS + 2 mg/L BA + 0.4 mg/L IAA +3% sucrose +.6 g/L agar. The rooting rate of test tube plantlets was over 70% on the medium of 1/4MS +0.5 mg/L IB A +2% sucrose +6 g/L agar.关键词
枣/嫩枝/茎段/组织培养/不定芽再生Key words
Jujube/ Shoots/ Stem segment/ Tissue culture/ Regeneration of adventitious buds分类
农业科技引用本文复制引用
孙洪雁,孙清荣,单公华,刘庆忠,周广芳..鲁枣1号组织培养和植株再生体系研究[J].山东农业科学,2012,44(1):14-16,3.基金项目
国家林业科技支撑计划(2008BAD92B03) (2008BAD92B03)
山东省农业良种工程项目(2008LZ010,2009LZ09) (2008LZ010,2009LZ09)
山东省农业科学院博士科研启动基金(2007YBS004) (2007YBS004)
泰安市科技发展计划(20093021) (20093021)