山东医药2012，Vol.52Issue(5)：25-28,4.
地塞米松对哮喘小鼠气道平滑肌细胞中胸腺活化调节趋化因子表达的影响
Influence of Dexamethasone on thymus activation regulated chemokine in asthma smooth muscle cells of asthmatic mice
摘要
Abstract
Objective To observe the influence of expression of dexamethasone on thymus activation regulated chemo-kine(TARC) in asthma smooth muscle cells in asthmatic mice models. Methods Thirty-six mice were randomly divided into three groups: normal control group ( group A), asthma model group ( group B) , and dexamethasone treated group (group C). The asthmatic mice models were established by OVA and AL(OH)3. White cells in BALF and eosinophils (EOS) were counted, HE-stained was used to observe the changes of pulmonary histopathology. The levels of TARC and IL-4 in both serum and BALF were measured by enzyme-linked immunosorbent assay (ELISA), then the expression of TARC protein in airway smooth muscle cells was assessed with immunohistochemistry. Results The total cell numbers and EOS count of group B were significantly higher than those in group A and group C(P<0.01), EOS counts of group C were higher than those of group A(P<0.05). The concentrations of TARC and IL-4 in serum of group B were obviously higher than that in group A and group C(P<0.01). The concentrations of TARC and IL-4 in BALF of group B were significantly higher than those in group A and group C (P < 0.01); the concentrations of TARC in BALF of group C were higher than those in group A(P <0.01). The protein expressions of TARC in airway smooth muscle cells of group B were significantly higher than those in group A and group C(P <0.01). In mice serum, strong positive correlations were found between TARC and IL-4(r=0.669, P < 0.01) ; there were significant positive correlations between TARC and IL4 in BALF (r=0.845,P<0.01). Conclusions The expressions of TARC in asthma smooth musde cells of asthma mice were significantly higher than those in normal control group. Dexamethasone may inhibit TARC expression through reducing secretion of IL-4.关键词
哮喘/胸腺活化调节趋化因子/地塞米松/小鼠Key words
asthma/ thymus activation regulated chemokine/ dexamethasone/ mouse分类
医药卫生引用本文复制引用
张娟,赵铭山..地塞米松对哮喘小鼠气道平滑肌细胞中胸腺活化调节趋化因子表达的影响[J].山东医药,2012,52(5):25-28,4.基金项目
山东省自然科学基金资助项目(Y2007C162). （Y2007C162）
