山东医药2012，Vol.52Issue(7)：12-14,3.
苦参素注射液联合顺铂对人肝癌SMMC-7721细胞凋亡相关基因c-myc、bcl-2和bax表达的影响
Influence of matrine injection combined with cisplatin on the expression of apoptosis associated genes c-myc, bcl-2 and bax in human hepatocarcinoma cell line SMMC-7721
摘要
Abstract
Objective To investigate the influence of matrine injection combined with cisplatin on the expression of apoptosis associated genes c-rayc, bcl-2 and bax in human hepatocarcinoma cell line SMMC-7721. Methods Apoptotic rate was determined by TUNEL method. The expression of c-myc, bcl-2 and bax mRNA were detected by semi-quantitive RT-PCR, and the expression of c-myc, bcl-2 and bax proteins were detected by two-step immunhistochemical staining. Results The apoptotic rate was increased significantly in matrine injection, cisplatin and combined agents group respectively, compared with the control group, the difference was statistically significant ( P < 0. 05 or P < 0. 01) . The effects of combined agents were simple addition or synergistic. The apoptotic rate was increased significantly, the expression of bax mRNA and protein was increased significantly, while c-myc, bcl-2 mRNA and proteins were decreased markedly in combined agents group, compared with cisplatin group, the difference was statistically significant ( P <0. 05 or P <0.01). Conclusion Matrine injection combined with cisplatin can obviously induce apoptosis of SMMC-7721 cells with simple addition or synergistic effects, and its mechanism may be involved in up-regulating expression of bax gene and down-regulating expression of c-myc and bcl-2 genes.关键词
苦参素注射液/顺铂/SMMC-7721细胞/细胞凋亡/基因表达Key words
matrine injection/ cisplatin/ SMMC-7721 cells/ apoptosis/ gene expression分类
医药卫生引用本文复制引用
韦星,黄赞松,黄岑汉,周喜汉,黄衍强,邓志华..苦参素注射液联合顺铂对人肝癌SMMC-7721细胞凋亡相关基因c-myc、bcl-2和bax表达的影响[J].山东医药,2012,52(7):12-14,3.基金项目
广西自然科学基金资助项目(0542119) （0542119）
广西高校优秀人才资助项目(桂教人20077029) （桂教人20077029）
广西卫生厅重点课题(重200887) （重200887）
广西教育厅课题(桂教科研200510197). （桂教科研200510197）
