山东医药2012，Vol.52Issue(7)：29-31,3.
PKC-a、K-ras蛋白在结直肠癌组织中的表达及意义
Expression and significance of PKC-a and K-Ras proteins in colorectal cancer
宋芳 1万义增 2陈素贤2
作者信息
- 1. 辽宁医学院研究生学院,辽宁锦州121000
- 2. 辽宁医学院附属第三医院
- 折叠
摘要
Abstract
Objective To investigate the roles of protein kinase C(PKC) and K-ras protein in the occurrence and development of colorectal cancer. Methods Immunohistochemistry method was used to detect the PKC-a and K-ras protein expression in 76 colorectal adenocarcinoma ( adenocarcinoma group) , 15 colorectal adenoma tissue (adenoma group) and 10 normal colorectal mucosa ( normal group) , their relationship and correlation with clinical pathological features of the tumor were analyzed. Western blot method was used to detect the PKC-a and K-ras protein expression level in 30 colorectal cancer tissues and 10 adjacent mucosa (away from the tumor 10 cm). Results The positive rate of PKC-a and K-ras in adenocarcinoma group was 35.5% and 68.4% , the former was significantly lower than that in normal mucosa, adenoma, while the later was on the opposite (all P <0. 05 ) . PKC-a and K-ras expression in colorectal carcinoma were correlated with clinical pathologic grading, with the increase of pathological grading, PKC-a expression rate reduced gradually, K-ras expression elevated; expression of the two indexes were not correlated with gender, age, lymph node metastasis and Dukes stage (P>0.05 ). PKC-a and K-ras protein expression was negatively correlated (r= -0.930). Conclusion PKC-a protein and K-ras protein are correlated with the development and progression of colorectal cancer.关键词
结直肠癌/蛋白激酶C/K-ras/免疫组化法/Western blotKey words
colorectal cancer / protein kinase C / K-Ras / immunohistochemical method / Western blot分类
医药卫生引用本文复制引用
宋芳,万义增,陈素贤..PKC-a、K-ras蛋白在结直肠癌组织中的表达及意义[J].山东医药,2012,52(7):29-31,3.
